Bioteke Corporation (Wuxi) Co., Ltd.  

Product Description Product Name Magnetic Bead Viral DNA/RNA Extraction Kit Specification 96 tests/kit Intended Use It is used to isolate and purify high-quality viral DNA / RNA from tissue, blood, serum, plasma, lymph fluid, acellular body fluids, cell culture supernatants or various virus preservation solutions. Test Principle During the nucleic acid extraction process, the magnetic bead adsorption principle is used to adsorb, transfer, and purify nucleic acids through special magnetic beads to automatically complete the nucleic acid extraction.Applicable instrument Suitable for AU1001-96 automatic nucleic acid extraction instrument Sample requirements Fresh samples. Experiment as soon as possible after sample collection. It can be stored temporarily at 2-8 ° C. If long-term storage, it should be stored at -20 ° C or -80 ° C. Instructions 1. Reagent preparation (40 mg/mL concentration of Proteinase K solution preparation) 1.1 Add 1 mL of deionized water into each 40 mg proteinase K dry powder to get a proteinase K solution with concentration of 40 mg/mL. 1.2 Add 500 uL of deionized water into 20 mg of proteinase K dry powder to get a proteinase K solution with concentration of 40 mg/mL. 1.3 Mix upside down and about 10 times to make sure the dry powder completely dissolved. The solution can be stored at 2-8 ° C for short-term use. If long-term storage, keep at -20 ° C. The number of repeated freeze-thaw cycles shall not exceed 5 times. 2. Sample preparation Tissue samples: Take 10 ~ 30 mg of liquid nitrogen and grind to a fine powder, transfer to a 1.5 mL centrifuge tube, add 200 μL of 1 X PBS buffer to resuspend, and then proceed to the next step. Sample Preservation Tube (with virus): no need to deal with, proceed directly to the next step 3. Pre-packaged deep well plate preparation 3.1 Carefully tear the plastic film of the pre-packaged deep-well plate and keep the deep-well plate stable. 3.2 Add 200uL of prepared sample to the No. 1 deep well plate (96 tests). If sample volume is not enough, add PBS buffer. Then add 25 uL of proteinase K solution (concentration 40 mg/mL). 3.3 Put this deep well plate into the extractor smoothly, and then insert the stirring sleeve into the card slot. 4. Procedure for setting up the extractor "Temperature setting": set the lysis temperature to 90 ° C and the elution temperature to 56 ° C. Aftersaving the settings, click "Run" to start the experiment.5. After the experiment is completed, transfer the DNA/RNA solution in the No. 6 deep-well plate to a clean nuclease-free centrifuge tube for direct downstream experiments or to be stored at -20 ºC